Culture medium
Published Time:
2025-02-05
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Cell culture media used for producing therapeutic proteins should have a precise nutrient balance to support their proliferation and growth. These nutrients are provided by the media, which must be sterile. The final sterilization process should ensure the removal of bacteria and mycoplasma from media containing animal-derived components. The media is then stored in fermenters or bioreactors after batch preparation and sterile filtration. Pre-filters are used to remove most particulate and colloidal contaminants from the media to extend the life of the final sterilizing filter.
Separation Objective
● Pre-filtration
Remove colloids, lipids, and particulates
● Sterile filtration
Remove bacteria and mycoplasma
Application Requirements
● Pre-filtration should effectively remove particulate and colloidal contaminants without hindering the passage of important components.
● The pre-filter must have a high flow rate and consistently high throughput to allow for timely and economical completion of the separation process. In addition, the filtration system should be compatible with variations caused by batch-to-batch differences in components.
Recommendation
| Filtration Steps | Recommendation |
| Pre-filtration | MCE, GF |
| Sterile Venting | PTFE |
| Final Filtration | PES & Multi-layered PES |
Large-volume injection process
None
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